Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Mapping and mutational analysis of the IgE-binding epitopes on Ara h 1, a legume vicilin protein and a major allergen in peanut hypersensitivity. Pistachio nut hypersensitivity: identification of pistachio nut allergens. Parra F M, Cuevas M, Lezaun A, Alonso M D, Beristain A M, Losada E. Allergy to pistachio: crossreactivity between pistachio nut and other Anacardiaceae.
Fiandor A, Martinez-Garate A, Martinez Quesada J. J Investig Allergol Clin Immunol 2000 10 173-7. Allergy to Anacardiaceae: description of cashew and pistachio nut allergens. Garcia F, Moneo I, Fernandez B, Garcia-Menaya J M, Blanco J, Juste S, Gonzalo J. Cohort study of peanut and tree nut sensitization by age of 4 years. Tariq S M, Stevens M, Matthews S, Ridout S, Twiselton R, Hide D W. Atopic dermatitis and food hypersensitivity reactions. Burks A W, James J M, Hiegel A, Wilson G, Wheeler J G, Jones S M, Zuerlein N. Marks J G Jr, DeMelfi T, McCarthy M A, Witte E J, Castagnoli N, Epstein WL, Aber R C. In addition, we identify several linear epitopes and compare these to the allergenic peanut vicilin, Ara h 1. Herein, we disclose the sequence of a cDNA encoding this allergenic polypeptide, designated Ana o 1, and describe the characterization of the expressed recombinant polypeptide. We have also identified a polypeptide allergen in the 7S superfamily, which includes vicilin-like and sucrose binding proteins. 5,6 Cashew nuts are widely used in snack foods and as an ingredient in a variety of processed foods such as bakery and confectionery products.īy sequencing of the N-termini and selected internal digests, we have found that the major allergenic proteins in cashew nut are legumin-like proteins and 2S albumins.
2-4 Pistachios are another allergenic member of the Anacardiaceae family and show extensive in vitro cross-reactivity with cashews. Most plant food allergens can be found among pathogenesis-related proteins, seed storage albumins and globulins, and a-amylase and protease inhibitors.Ĭashew nuts have been associated with contact or systemic dermatitis (to cardol and anacardic acid found in the cashew nut shell oil 1), atopic dermatitis, and IgE-mediated systemic allergic reactions. While food-induced allergic reactions are the most common cause of outpatient anaphylaxis, the majority of severe reactions of this kind are caused by peanuts and tree nuts. It is estimated that up to 8% of children less than 3 years of age and 2% of adults are affected by food allergies.
The present invention relates to the field of allergies to nuts and, more particularly, to purified linear epitope polypeptides from cashews, nucleic acids encoding therefor, and associated methods. 2009, which CRF copy is incorporated herein by reference in its entirety. This application includes a “Sequence Listing” printed on paper and a copy thereof in computer readable format (CRF copy) submitted on a Compact Disk-Recordable (CD-R) containing one file named 12025875-SEQS.ST25, which is 16 KB in size and was recorded on 10 Jun. 11, 2002, each priority application being incorporated herein by reference in its entirety. 6,884,877, and which claimed priority from provisional application Ser. 7,381,534, which claimed priority from and was a continuation of Ser. This application is a division of and claims priority from utility patent application Ser.
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